人脐带间充质干细胞描述:
间充质干细胞(MSC)是一种特征明确的成体干细胞群体。间充质干细胞有潜力发育成产生脂肪、软骨、骨骼、肌腱和肌肉的成熟细胞。由于 MSC 在再生医学中的潜在用途,这些特性与其发育可塑性相结合,引起了极大的兴趣。使用神经元条件培养基、音刺猬和FGF-8,在体外诱导从脐带华顿氏胶中分离的MSC转化为神经元和神经胶质细胞。MSC 还可以分化为脂肪形成和成骨谱系的细胞。此外,通过在心肌细胞条件培养基中培养,它们有可能分化成心肌细胞。MSC 表达基质受体 CD44 和 CD105 以及间充质干细胞标记物 SH2 和 SH3,
ScienCell 研究实验室的 HUMSC 是从脐带沃顿氏胶中分离出来的。HUMSC 在第一代中进行冷冻保存并冷冻交付。每个小瓶在 1 ml 体积中含有 >5 x 105。HUMSC 的特征在于使用 CD73 和/或 CD90 特异性抗体进行免疫荧光、成脂分化后的油红 O 染色以及成骨分化后的茜素红染色。HUMSC 对 HIV-1、HBV、HCV、支原体、细菌、酵母和真菌呈阴性。在ScienCell研究实验室提供的条件下,HUMSC保证进一步扩增15倍增。
货号 | 7530 |
产地 | 美国 |
缩写 | HUMSC |
规格 | 5 x 10^5 cells/vial |
用途 | 科研 |
运输 | 干冰 |
保存 | 液氮 |
1.) Liu J, Xu HH, Zhou H, Weir MD, Chen Q, Trotman CA. (2013) "Human umbilical cord stem cell encapsulation in novel macroporous and injectable fibrin for muscle tissue engineering." Acta Biomater. 9: 4688-97.
2.) Lee HK, Finniss S, Cazacu S, Bucris E, Ziv-Av A, Xiang C, Bobbitt K, Rempel SA, Hasselbach L, Mikkelsen T, Slavin S, Brodie C. (2013) "Mesenchymal stem cells deliver synthetic microRNA mimics to glioma cells and glioma stem cells and inhibit their cell migration and self-renewal." Oncotarget. 4: 346-61.
3.) Chen W, Zhou H, Tang M, Weir MD, Bao C, Xu HH. (2012) "Gas-foaming calcium phosphate cement scaffold encapsulating human umbilical cord stem cells." Tissue Eng Part A. 18: 816-27.
4.) Liu J, Zhou H, Weir MD, Xu HH, Chen Q, Trotman CA. (2012) "Fast-degradable microbeads encapsulating human umbilical cord stem cells in alginate for muscle tissue engineering." Tissue Eng Part A. 18: 2303-14.
5.) Tang M, Weir MD, Xu HH. (2012) "Mannitol-containing macroporous calcium phosphate cement encapsulating human umbilical cord stem cells." J Tissue Eng Regen Med. 6: 214-24.
6.) Theinhan W, Weir MD, Simon CG, Xu HH. (2012) "Non-rigid calcium phosphate cement containing hydrogel microbeads and absorbable fibres seeded with umbilical cord stem cells for bone engineering." J Tissue Eng Regen Med. doi: 10.1002/term.1466.
7.) Zhou H, Chen W, Weir MD, Xu HH. (2012) "Biofunctionalized calcium phosphate cement to enhance the attachment and osteodifferentiation of stem cells released from fast-degradable alginate-fibrin microbeads." Tissue Eng Part A. 18: 1583-95.
8.) Thein-Han W, Xu HH. (2011) "Collagen-calcium phosphate cement scaffolds seeded with umbilical cord stem cells for bone tissue engineering." Tissue Eng Part A. 17: 2943-54.
9.) Zhao Y, Yu L, Xu S, Qiu F, Fan Y, Fu G. (2011) "Down-regulation of connexin43 gap junction by serum deprivation in human endothelial cells was improved by (-)-Epigallocatechin gallate via ERK MAP kinase pathway." Biochem Biophys Res Commun. 404: 217-22.
10.) Zhao Z, Chen Z, Zhao X, Pan F, Cai M, Wang T, Zhang H, Lu JR, Lei M. (2011) "Sphingosine-1-phosphate promotes the differentiation of human umbilical cord mesenchymal stem cells into cardiomyocytes under the designated culturing conditions." J Biomed Sci. 18: 37.
11.) Zhou H, Weir MD, Xu HH. (2011) "Effect of cell seeding density on proliferation and osteodifferentiation of umbilical cord stem cells on calcium phosphate cement-fiber scaffold." Tissue Eng Part A. 17: 2603-13.
12.) Zhou H, Xu HH. (2011) "The fast release of stem cells from alginate-fibrin microbeads in injectable scaffolds for bone tissue engineering." Biomaterials. 32: 7503-13.
13.) Peng J, Wang Y, Zhang L, Zhao B, Zhao Z, Chen J, Guo Q, Liu S, Sui X, Xu W, Lu S. (2011) "Human umbilical cord Wharton's jelly-derived mesenchymal stem cells differentiate into a Schwann-cell phenotype and promote neurite outgrowth in vitro." Brain Res Bull. 84: 235-43.
14.) Zhao L, Burguera EF, Xu HH, Amin N, Ryou H, Arola DD. (2010) "Fatigue and human umbilical cord stem cell seeding characteristics of calcium phosphate-chitosan-biodegradable fiber scaffolds." Biomaterials. 31: 840-7.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
微信公众号
微信客服