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人脑微血管内皮细胞 (Sciencell进口)
人脑微血管内皮细胞 (Sciencell进口)
人脑微血管内皮细胞 (Sciencell进口)
人脑微血管内皮细胞 (Sciencell进口)
人脑微血管内皮细胞 (Sciencell进口)

人脑微血管内皮细胞 (Sciencell进口)

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  • 英文名:Human Brain Microvascular Endothelial Cells
  • 规 格:5 x 10^5 cells/vial
  • 货 号:1000
  • 价 格: 询价
数 量: - +
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人脑微血管内皮细胞 (Sciencell进口)
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    人脑微血管内皮细胞描述:


         脑微血管内皮细胞(BMEC)是血脑屏障的主要组成部分,它限制了可溶性物质和细胞物质从血液进入大脑。BMEC具有与周围内皮细胞不同的独特特征,如1)细胞间紧密连接显示出高电阻和缓慢的细胞旁通量,2)没有窗窗和降低的胞内活动水平,以及3)不对称定位的酶和载体介导的运输系统。与外周内皮细胞类似,BMEC在其表面表达或可诱导表达细胞粘附分子,调节白细胞向脑外渗。BMEC因其独特的分子和细胞特性而被广泛用于研究血脑屏障的分子和细胞特性

    功能。


         来自ScienCell研究实验室的HBMEC是从人脑中分离出来的。HBMEC在传代一时冷冻保存,冷冻递送。每个小瓶含有>5 × 10^5个细胞,体积为1ml。HBMEC的特征是免疫荧光特异性抗体vWF和/或CD31 (PECAM1)。HBMEC对HIV-1、HBV、HCV、支原体、细菌、酵母和真菌呈阴性。保证HBMEC在ScienCell研究实验室提供的条件下进行进一步培养;然而,由于扩展能力有限,HBMEC不推荐用于扩展或长期培养。


    推荐培养基:

         推荐使用内皮细胞培养基(ECM, Cat。#1001)用于体外培养HBMEC。

          

    产品使用说明:仅供科研研究使用!

    货号

    1000

    产地

    美国

    缩写

    HBMEC

    规格

    5 x 10^5 cells/vial

    用途

    科研

    运输

    干冰

    保存

    液氮

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    17.) Cucullo L, Hossain M, Puvenna V, Marchi N, Janigro D. (2011) "The role of shear stress in Blood-Brain Barrier endothelial physiology." BMC Neurosci. 12: 40.

    18.) Feng S, Cen J, Huang Y, Shen H, Yao L, Wang Y, Chen Z. (2011) "Matrix metalloproteinase-2 and -9 secreted by leukemic cells increase the permeability of blood-brain barrier by disrupting tight junction proteins." PLoS One. 6: e20599.

    19.) Grau S, Thorsteinsdottir J, von Baumgarten L, Winkler F, Tonn JC, Schichor C. (2011) "Bevacizumab can induce reactivity to VEGF-C and -D in human brain and tumour derived endothelial cells." J Neurooncol. 104: 103-12.

    20.) G?bel K, Pankratz S, Schneider-Hohendorf T, Bittner S, Schuhmann MK, Langer HF, Stoll G, Wiendl H, Kleinschnitz C, Meuth SG. (2011) "Blockade of the kinin receptor B1 protects from autoimmune CNS disease by reducing leukocyte trafficking." J Autoimmun 36(2): 106-114.

    21.) Gandhi N, Saiyed ZM, Napuri J, Samikkannu T, Reddy PV, Agudelo M, Khatavkar P, Saxena SK, Nair MP. (2010) "Interactive role of human immunodeficiency virus type 1 (HIV-1) clade-specific Tat protein and cocaine in blood-brain barrier dysfunction: implications for HIV-1-associated neurocognitive disorder." J Neurovirol. 16: 294-305.

    22.) Lin CJ, Tai Y, Huang MT, Tsai YF, Hsu HJ, Tzen KY, Liou HH. (2010) "Cellular localization of the organic cation transporters, OCT1 and OCT2, in brain microvessel endothelial cells and its implication for MPTP transport across the blood-brain barrier and MPTP-induced dopaminergic toxicity in rodents." J Neurochem. 114: 717-27.

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    24.) Patel NS, Mathura VS, Bachmeier C, Beaulieu-Abdelahad D, Laporte V, Weeks O, Mullan M, Paris D. (2010) "Alzheimers beta-amyloid peptide blocks vascular endothelial growth factor mediated signaling via direct interaction with VEGFR-2." J Neurochem. 112: 66-76.

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    27.) Ghosh C, Gonzalez-Martinez J, Hossain M, Cucullo L, Fazio V, Janigro D, Marchi N. (2010) "Pattern of P450 expression at the human blood-brain barrier: roles of epileptic condition and laminar flow." Epilepsia. 51: 1408-417.

    28.) Rosch JW, Boyd AR, Hinojosa E, Pestina T, Hu Y, Persons DA, Orihuela CJ, Tuomanen EI. (2010) "Statins protect against fulminant pneumococcal infection and cytolysin toxicity in a mouse model of sickle cell disease." J Clin Invest. 120: 627-35.

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    34.) Cucullo L, Couraud PO, Weksler B, Romero IA, Hossain M, Rapp E, Janigro D. (2008) "Immortalized human brain endothelial cells and flow-based vascular modeling: a marriage of convenience for rational neurovascular studies." J Cereb Blood Flow Metab. 28: 312-28.

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